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KMID : 0923620130130060283
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Immune Network
2013 Volume.13 No. 6 p.283 ~ p.288
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BIRB 796 has Distinctive Anti-inflammatory Effects on Different Cell Types
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Ryoo So-Yoon
Choi Ji-Da
Kim Jae-Myung
Bae Su-Young
Hong Jae-Woo
Jo Seung-Hyun
Kim Soo-Hyun
Lee Young-Min
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Abstract
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The pro-inflammatory cytokines tumor necrosis factor-? (TNF?) and interleukin (IL)-1? are crucial mediators in-volved in chronic inflammatory diseases. Inflammatory signal pathways regulate inflammatory cytokine expression-medi-ated by p38 mitogen activated protein kinase (p38MAPK). Therefore, considerable attention has been given to p38MAPK as a target molecule for the development of a novel anti-in-flammatory therapeutics. BIRB 796, one of p38MAPK in-hibitor, is a candidate of therapeutic drug for chronic in-flammatory diseases. In this study, we investigated the ef-fect of BIRB 796 on inflammatory cytokine productions by lipopolysaccharide (LPS) in different immune cell types. BIRB 796 reduced LPS-mediated IL-8 production in THP-1 cells but not in Raw 264.7 cells. Further analysis of signal molecules by western blot revealed that BIRB 796 sufficiently suppressed LPS-mediated phosphorylation of p38MAPK in both cell types whereas it failed to block inhibitor of kappa B (I-?B) degradation in Raw 264.7 cells. Taken together, these results suggest that the anti-inflammatory function of BIRB 796 depends on cell types.
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KEYWORD
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p38MAPK, BIRB 796, Inflammatory cytokine, Inflammatory signal pathways, LPS
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